Identification of Chiral-Specific Carbon Nanotube Binding Peptides Using a Modified Biopanning Method
نویسندگان
چکیده
Peptides can recognize and selectively bind to a wide variety of materials dependent on both their surface properties the environment. Biopanning with phage or cell peptide display libraries identify material-specific binding peptides. However, limitations sequence diversity traditional bacteriophage (phage) loss unique clones during amplification cycles results in smaller pool sequences identified. False positive tend emerge biopanning process due highly proliferating, yet nonspecific, phages. In order overcome this limitation methodology, modified method using high-throughput next generation sequencing (HTS) was tested select for peptides specific two types single wall carbon nanotube (SWNTs) sources varying diameter distribution chirality. Here, process, analysis, characterization identified is further described compared literature method. Selected from study were incorporated SWNT dispersion experiment probe selectivity diameter. We show that NHTS uncover might have otherwise been lost
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2021
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors9090245